Microwave spectroscopic detection of human Hsp70 protein on annealed gold nanostructures on ITO glass strips - Université de technologie de Troyes Access content directly
Journal Articles Biosensors Year : 2018

Microwave spectroscopic detection of human Hsp70 protein on annealed gold nanostructures on ITO glass strips

Abstract

Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm2 of Hsp70.

Dates and versions

hal-02365657 , version 1 (15-11-2019)

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Cite

Rodica Elena Ionescu, Raphael Selon, Nicolas Pocholle, Lan Zhou, Anna Rumyantseva, et al.. Microwave spectroscopic detection of human Hsp70 protein on annealed gold nanostructures on ITO glass strips. Biosensors, 2018, 8 (4), pp.1-10. ⟨10.3390/bios8040118⟩. ⟨hal-02365657⟩
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