Nanometer-Scale Resolution Achieved with Nonradiative Excitation - Université de technologie de Troyes Access content directly
Journal Articles ACS photonics Year : 2018

Nanometer-Scale Resolution Achieved with Nonradiative Excitation


Nonradiative Excitation Fluorescence Microscopy (NEFM) is a promising technique allowing the observation of biological samples beyond the diraction limit. By coating a substrate with an homogeneous monolayer of quantum dots (QDs), NEFM is achieved through a nonradiative energy transfer from QDs (donors) to dye molecules located in the sample (acceptors). The excitation depth of the sample is then given by the För-ster radius, which corresponds to few nanometers above the surface. The powerful axial resolution of NEFM is highlighted by observing the adhesion of Giant Unilamellar Vesi-cles (GUVs) on strong interaction with coated surfaces. In this paper, we demonstrate that the QD-quenching level is valuable to calculate and map the distance between the membrane and the surface with a high precision along the optical axis. By tuning the electrostatic interactions between the membrane and the substrate, we have been able to measure a height displacement of ≈ 1 nm of the lipid membrane. The experimental results were discussed according to simulations, which take into account all the common forces appearing between lipid membranes and surfaces.
Fichier principal
Vignette du fichier
acs_photo_vezy_final.pdf (8.41 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-02363346 , version 1 (14-11-2019)



Lina Riachy, Dalia El Arawi, Rodolphe Jaffiol, Cyrille Vézy. Nanometer-Scale Resolution Achieved with Nonradiative Excitation. ACS photonics, 2018, 5 (6), pp.2217-2224. ⟨10.1021/acsphotonics.8b00063⟩. ⟨hal-02363346⟩
29 View
155 Download



Gmail Facebook X LinkedIn More