The process of optical fiber probe production was introduced in this paper, as well as the attachment of silver nanoparticles, the enhanced effect of silver nanoparticles in fiber-optic probe inhomogeneous electromagnetic field of was simulated using the finite difference time domain method. Firstly, the electric field distribution of different shapes of the fiber polymer probe tip was discussed, and it provided a reference for the research of nanoparticles polarization effects; Secondly, the polarization affecting factors of single nanoparticle were simulated, the radius of silver nanoparticle and its distance to the probe were taken into consideration; Finally, taking two silver nanoparticles for example, the polarization effect of particles relative position were discussed,which proved that the silver nanoparticles outside the top of the fiber probe do not contribute to the polarization effect. The simulation results of this paper provided a theoretical support for the production of the fiber-optic probe and the attachment of metal nanoparticles.